We propose in this paper a quantization scheme for real Klein-Gordon field in de Sitter spacetime. Our scheme is generally covariant with the help of vierbein, which is necessary usually for spinor field in curved spacetime... We first present a Hamiltonian structure, then quantize the field following the standard approach. For the free field, the time-dependent quantized Hamiltonian is diagonalized by Bogliubov transformation and the eigen-states at each instant are interpreted as the observed particle states at that instant. The interpretation is supported by the known cosmological red-shift formula and the on-shell condition of 4-momentum for a free field. Though the mathematics is carried out in term of conformal coordinates for the sake of convenience, the whole theory can be transformed into any other coordinates based on general covariance. It is concluded that particle states, such as vacuum states in particular are time-dependent and vacuum states at one time evolves into non-vacuum states at later times. Formalism of perturbational is provided with en extended Dirac picture. (read more)